Penelitian ini bertujuan menganalisis pengaruh perceived organizational support (POS), kepemimpinan, dan beban kerja terhadap stres kerja pegawai SAMSAT Kabupaten Kuningan secara parsial maupun simultan. Latar belakang penelitian didasarkan pada meningkatnya volume pekerjaan, ditandai lonjakan tunggakan kendaraan hingga 115.861 unit (34,44%) pada 2025, serta hasil pra-survei yang menunjukkan 84,4% pegawai mengalami kelelahan fisik dan emosional. Metode yang digunakan adalah kuantitatif dengan pendekatan survei dan teknik total sampling. Data dikumpulkan melalui kuesioner agree-disagree scale, kemudian dianalisis menggunakan regresi linear berganda, uji T, dan uji F. Hasil penelitian menunjukkan: (1) perceived organizational support berpengaruh negatif dan signifikan terhadap stres kerja, semakin tinggi dukungan organisasi, semakin rendah stres pegawai. (2) kepemimpinan berpengaruh negatif dan signifikan, semakin baik kualitas kepemimpinan, semakin rendah stres kerja. (3) beban kerja berpengaruh positif dan signifikan, semakin tinggi beban kerja, semakin tinggi stres yang dirasakan. (4) ketiga variabel secara simultan berpengaruh signifikan terhadap stres kerja pegawai SAMSAT Kabupaten Kuningan.

This study aimed to analyze the effect of perceived organizational support (POS), leadership, and workload on job stress among employees of SAMSAT Kuningan Regency, both partially and simultaneously. The study was grounded in a rising work volume phenomenon, marked by a surge in vehicle tax arrears reaching 115,861 units (34.44%) in 2025, alongside pre-survey findings that indicated 84.4% of employees experienced physical and emotional exhaustion from work pressure. A quantitative survey approach was employed, with data collected from the entire employee population using total sampling. Data were gathered through an agree-disagree scale questionnaire and analyzed using multiple linear regression, T-test, and F-test. The results revealed that (1) POS had a negative and significant effect on job stress, (2) leadership had a negative and significant effect on job stress, (3) workload had a positive and significant effect on job stress, (4) all three variables simultaneously had a significant effect on job stress among employees of SAMSAT Kuningan Regency.